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WebOne-Step Coating Procedure. Wash Dynabeads® MyOne™ Carboxylic Acid twice with 25mM MES, pH 6, using the equal volume of Dynabeads® (300 μl) pipetted out of the vial, for ten minutes with good mixing (en-over-end or similar). Add the required amount of ligand in 25 mM MES, pH 6 (60 μl) to the washed Dynabeads® . WebFeb 10, 2012 · Probably hadn't been properly broken in. I cleaned it to bare metal with solvents and JB Bore cleaner, then did the Dyna Bore Coat treatment. Copper cleans with a swipe or two with a patch and Boretech Eliminator. Carbon still shows some build up and occasionally needs JB scrubbing for a few inches ahead of the chamber. how many hours is fasting for lab work
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