WebAug 2, 2024 · Approximately 72 h post-infection, fresh culture medium supplemented with 30% fetal bovine serum and 1.0 μg/ml puromycin was used to culture the lentivirus-infected target HCC cells. Only cells contacting viral particles were able to survive and propagate under the puromycin selection. WebOct 30, 2024 · The Cytoscape software was applied to evaluate the interactive relationships between the candidate proteins. Afterward, two clusters consist of 65 nodes and 23 …
Identifying of biomarkers associated with gastric cancer based …
WebMay 12, 2024 · A protein-protein interaction (PPI) network was constructed using Cytoscape v3.7.0. The hub genes were selected using the Maximal Clique Centrality (MCC) method. A circRNA-miRNA-hubgene (ceRNA) network was constructed using Cytoscape v3.7.0. Results CircRNA expression profile in plasma of OC and control patients WebNov 20, 2024 · The hub gene of top 100 was obtained by MCC method of cytohubb and were imported into Cytoscape to construct the protein–protein interaction (PPI) network. In addition, we used R package clusterProfiler (version 3.16.1) [ 26 ] to analyze the statistical enrichment of miRNA-target genes in GO and KEGG. derrick streaming
Identification of potential candidate genes for lip and oral …
WebJul 2, 2024 · (B) Top 10 hub genes calculated by the MCC method in CytoHubba software. The gradation of color represents the value of the score according to the MCC method, and the VEGFA score was the … WebIn a co-expression network, Maximal Clique Centrality (MCC) algorithm was reported to be the most effective method of finding hub nodes . The MCC of each node was calculated … WebDec 5, 2024 · Hub genes were identified using the cytoHubba Cytoscape plugin, and maximal clique centrality (MCC) was identified. The MCODE Cytoscape plugin was used to identify network clusters, or highly interconnected regions. RESULTS There were 586 and 392 DEGs identified from the GSE103176 and GSE54644 datasets, respectively. derrick storm shorts