Biotinylated cytidine bis phosphate
WebBiotinylated cytidine (Bis)phosphate (1 mM). 4. RNase inhibitor (40 U/μl). 5. ... This procedure ligates biotin-labeled cytidine (bis) phosphate nucleotide that contains a 3′, 5′ phosphate on the ribose ring to accommodate ligation of the cytidine with the biotin linker. The ligation should be performed in a nuclease-free tube on a ...
Biotinylated cytidine bis phosphate
Did you know?
WebThe RNA 3' End Biotinylation Kit uses T4 RNA ligase to conjugate a single nucleotide analog to the 3' terminus of an RNA strand. The unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with > 70% efficiency. WebNational Center for Biotechnology Information
WebBis (p-nitrophenyl) phosphate sodium salt Linear Formula: C12H8O8N2PNa CAS No.: 4043-96-3 Molecular Weight: 362.16 EC No.: 223-739-2 Match Criteria: Keyword All Photos ( 1) Bis (4-nitrophenyl) phosphate hydrate Synonym (s): … WebDesthiobiotinylated Cytidine (Bis)phosphate, (1mM), 40µL . Glycogen (20mg/mL), 20µL . Storage: Upon receipt store at -20°C. Product shipped on dry ice. Introduction ... Biotinylated Cytidine Bisphosphate 1 1nmol T4 RNA Ligase 2 40U PEG 30% 15 15% Total 30 --- 5. Incubate the reactions at 16°C for 2 hours for the control RNA.
WebMar 28, 2016 · Biotinylated cytidine (bis)phosphate (pCp-Biotin; Jena Bioscience, cat. no. NU-1706-BIO) Proteinase K, molecular biology grade, 0.8 U/μl (New England BioLabs, cat. no. P8107S) WebApr 1, 2014 · This protocol is used to radiolabel the 3' ends of RNAs, either synthesized by in vitro transcription or purified from cells or tissues, by ligation of [5'- (32)P]cytidine 3',5' …
WebPCP4/PEP-19 antibody (Biotin) [orb445051] Sizes Available: 100 μl BMP1 antibody (Biotin) [orb446168] Sizes Available: 100 μl PRCP antibody (Biotin) [orb114326] Sizes Available: 100 μl PCP antibody [orb818418] Sizes Available: 10 mg BMP1 antibody [orb859001] Sizes Available: 10 mg
WebMar 22, 2024 · After permeabilization and micrococcal nuclease (MNase) treatment, the RNA fragments in close proximity are labeled with biotinylated cytidine (bis) phosphate (pCp-biotin) and ligated in situ. The cells are lysed and then incubated with a specific antibody to pull-down the RBP-organized full complement of RNA-RNA spatial contacts ( … small home photography studioWebThe unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with > 70% efficiency. Labeling … sonic christmas coloring sheetsWebVisit ChemicalBook To find more CYTIDINE 3',5'-BIS(PHOSPHATE), [5'-32P]-() information like chemical properties,Structure,melting point,boiling point,density,molecular formula,molecular weight, physical properties,toxicity information,customs codes. sonic chromatic fnfWebLong non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides (nt) that are not translated into known functional proteins. This broad definition covers a large collection of transcripts with diverse genomic origins, biogenesis, and modes of action. Thus, it is very important to choose appropriate research methodologies when investigating lncRNAs … sonic christian whitehead pc portWebMeaning of biotinylated. What does biotinylated mean? Information and translations of biotinylated in the most comprehensive dictionary definitions resource on the web. sonic chromeWebThe RNA 3' End Biotinylation Kit uses T4 RNA ligase to conjugate a single nucleotide analog to the 3' terminus of an RNA strand. The unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with greater than 70% efficiency. … small home plans for disabledWebS. Muller, in Laboratory Techniques in Biochemistry and Molecular Biology, 1999 2.10.2 Biotinylation of peptides. Biotinylation of peptides has a number of applications. Since … small home pictures